The effect of storage condition on seed germination of six Hydrocharitaceae and Potamogetonaceae species

Seed sources are vital for restoration and conservation of submerged macrophytes. Developing proper storage techniques is important to maintain seed vigor. The present study aimed to investigate the appropriate seed storage methods for species of Hydrocharitaceae (Ottelia acuminata, O. alismoides, and Valisneria natans) and Potamogetonaceae (Potamogeton lucens, P. wrightii, and Stuckenia pectinata). Seed germination was tested for these six species stored for 30 months in seven conditions (4 °C-Water, 4 °C-Dry, 4 °C-Sand, Ambient-Water, Ambient-Dry, Ambient-Sand, and ambient storage condition: submerged in a pond with lake water). Our results showed that: 1) the three Hydrocharitaceae species had high germination in ambient storage condition; 2) that the germination of these six species was low in ambient temperature storage conditions (Ambient-Water, Ambient-Dry, and Ambient-Sand); 3) that O. acuminata, V. natans, P. wrightii and S. pectinata had higher germination percentage in 4 °C-Dry than in 4 °C-Water. These results suggest that low temperature and dry conditions are favorable to maintain most species seed viability. Natural condition is appropriate for Hydrocharitaceae seed storage. This study will help the conservation and restoration of submerged macrophytes.