Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)

Our results indicated a decrease in response to stimulus and a decrease in OpR that were associated with increased concentrations of MS-222. This may assist in establishing anaesthetic protocols using MS-222 in fish and supports the use of supramaximal pressure stimuli to teleost fish under variable MS-222 concentrations as a model for future studies.