Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5789989 | Livestock Science | 2015 | 37 Pages |
Abstract
The liver is an important organ responsible for amino acids (AA) conversion, and catabolism. To investigate the metabolic characteristic of AA in liver, eighteen castrated male pigs (20±1 kg), implanted with arterial-portal-hepatic-mesenteric catheters, were fed three crude protein (CP) diets (14%, 17%, 20%) with six pigs per group. Blood was sampled for 7.5 h post-feeding. Hepatic tissue was sampled from additional fifteen pigs (three CP diets groups, 5 per group) on 3 h post-feeding. Portal vein plasma flow (PVPF), hepatic vein plasma flow (HVPF), hepatic cumulative net appearance and related gene expression were analyzed. There were no difference (P>0.05) for PVPF and HVPF among the three CP diets. Compared with the 20% CP diet, the 14% CP diet increased (P<0.05) the hepatic metabolism of lysine (59.50% vs. 43.00%) and threonine (44.30% vs. 30.22%). Likewise, the mRNA levels of α-aminoadipate δ-semialdehyde synthase (AASS) and l-threonine 3-dehydrogenase (TDH) were highest (P<0.05) in 14% CP group. The branched-chain AA (BCAA) had a low metabolic rate (3.4-13.4%) in liver. Morever, both the branched-chain amino-transferase 2 (BCAT2) and branched-chain α-keto acid dehydrogenase (BCKDHA) genes had a low expression. In contrast, aromatic AA (AAA) were almost entirely metabolized (63-93%) and the genes of phenylalanine hydroxylase (PAH) and tyrosine aminotransferase (TAT) had a high expression in liver. Notably, compared with the 20% CP diet, the 14% CP diet increased (P<0.05) hepatic metabolism of BCAA, AAA and gene expressions of BCAT, BCKDHA, PAH and TAT. These results revealed pigs fed on a low CP diets, only supplemented with lysine, methionine, threonine and tryptophan, had a numerical increasing EAA metabolism and EAA related metabolic gene expression in liver.
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Authors
L. Li, P. Zhang, P. Zheng, Z. Bao, Y. Wang, F.R. Huang,