Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5790017 | Livestock Science | 2015 | 9 Pages |
Abstract
The effects of antioxidants from flax meal (FM) and abomasal infusion of flax oil (FO) on the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase (GPX)) in blood and mammary tissue and the mRNA abundance of antioxidant and lipogenic-related genes in mammary tissue of dairy cows were determined. Eight ruminally fistulated lactating Holstein cows were assigned to a double 4Ã4 Latin square design with a 2Ã2 factorial arrangement of treatments: (1) control diet with no FM (CON); (2) diet containing 124Â g/kg FM (FMD) in the dry matter (DM); (3) CON and 250Â g FO/d infused in the abomasum; (4) FMD and 250Â g FO/d infused in the abomasum. Catalase activity in erythrocytes tended to increase when cows were fed FMD. Abomasal infusion of FO had no effect on activity and gene expression of antioxidant enzymes and gene expression of lipogenic-genes in mammary tissue, except for an increase in GPX1 expression in the absence of FM. The results suggest that feeding 124Â g/kg FM and infusing 250Â g of FO/d in the abomasum of dairy cows does not induce significant changes in the activity of antioxidant enzymes in blood and mammary tissue, and expression of antioxidant and lipogenic-genes in mammary tissue. However, more studies are required to determine any beneficial effects of natural antioxidants such as FM on the oxidative status of cows supplemented with polyunsaturated fatty acids, which could lead to feeding strategies to prevent diseases affecting the health status of dairy cattle.
Keywords
SCDCD36GAPDHPPARαGPXstearoyl-CoA desaturaseNrf2FMDLPLCATPPIAACACApeptidylprolyl isomerase AUBQSREBP1acetyl-coenzyme A carboxylase alphaROSperoxisome proliferator-activated receptor alphaAntioxidantsactin betaPolyunsaturated fatty acidsfatty acid synthasePUFAOmega-3concluster of differentiation 36Flax oilcontrol dietSODSuperoxide dismutasesterol regulatory element binding transcription factor 1Actbnuclear factor (erythroid-derived 2)-like 2mRNA abundanceFasnFlaxseedLipoprotein lipaseLignansdry matterReference geneCatalaseglutathione peroxidaseglyceraldehyde-3-phosphate dehydrogenaseReactive oxygen speciesUbiquitin
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Authors
L.S. Lima, M.F. Palin, G.T. Santos, C. Benchaar, H.V. Petit,