| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5793233 | Preventive Veterinary Medicine | 2015 | 4 Pages |
Abstract
The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
Rafael Pereira Heckler, Dyego Gonçalves Lino Borges, Flávia Barbieri Bacha, Marcel Kenzo Vilalba Onizuka, LetÃcia e Silva Teruya, Juliana Paniago Lordelo Neves, Cássia Rejane Brito Leal, Ricardo Antônio Amaral de Lemos, Marcelo Vasconcelos Meireles,