Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5794546 | Research in Veterinary Science | 2016 | 7 Pages |
â¢Feline infectious peritonitis (FIP) is a coronavirus-induced fatal disease in cats.â¢We investigated therapeutic effect of anti-fTNF-α mAb for experimental FIP infection.â¢Anti-fTNF-α mAb improved the FIP symptoms and survival rate in 2 of 3 cats.â¢Anti-fTNF-α mAb improved plasma AGP and VEGF level and lymphopenia.â¢The results suggested the anti-fTNF-α mAb may be effective for the treatment of FIP.
Feline infectious peritonitis virus (FIPV) replication in macrophages/monocytes induced tumor necrosis factor (TNF)-alpha production, and that the TNF-alpha produced was involved in aggravating the pathology of FIP. We previously reported the preparation of a feline TNF-alpha (fTNF-alpha)-neutralizing mouse monoclonal antibody (anti-fTNF-alpha mAb). This anti-fTNF-alpha mAb 2-4 was confirmed to inhibit the following fTNF-alpha-induced conditions in vitro. In the present study, we investigated whether mAb 2-4 improved the FIP symptoms and survival rate of experimentally FIPV-inoculated SPF cats. Progression to FIP was prevented in 2 out of 3 cats treated with mAb 2-4, whereas all 3 cats developed FIP in the placebo control group. Plasma alpha1-glycoprotein and vascular endothelial growth factor levels were improved by the administration of mAb 2-4, and the peripheral lymphocyte count also recovered. These results strongly suggested that the anti-fTNF-alpha antibody is effective for the treatment of FIP.