Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5794828 | Research in Veterinary Science | 2014 | 13 Pages |
â¢T. spiralis Ag30 was fused to three copies of the molecular adjuvant P28.â¢Ag30-P283 was either expressed on the surface or secreted by attenuated Salmonella.â¢Secreted Ag30-P283 induced high protection against T. spiralis challenge (92.8%).â¢Protection was associated with a Th2 type of immune response.
The development of a veterinary vaccine against T. spiralis infection is an alternative strategy to control trichinellosis. In an effort to develop an efficient vaccine, BALB/c mice were immunized with attenuated Salmonella enterica serovar Typhimurium SL3261 that expresses a 30-mer peptide (Ag30) derived from the gp43 of T. spiralis muscle larvae fused to three copies of the molecular adjuvant P28 (Ag30-P283) and it was either displayed on the surface or secreted by recombinant Salmonella strains. Salmonella strain secreting Ag30-P283, reduced the adult worm burden 92.8% following challenge with T. spiralis muscle larvae compared to 42% achieved by recombinant Salmonella displaying Ag30-P283 on the surface. The protection induced by secreted Ag30-P283 was associated with a mixed Th1/Th2 with predominance of Th2 phenotype, which was characterized by the production of IgG1, intestinal IgA antibodies and IL-5 secretion. This finding could provide an efficient platform technology for the design of novel vaccination strategies.
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