Article ID Journal Published Year Pages File Type
5795387 Small Ruminant Research 2016 8 Pages PDF
Abstract

•Dromedary camel semen was subjected to liquefaction by high-power low frequency ultrasound exposure.•Semen rheological and physical properties were evaluated throughout the period of exposure to ultrasound waves.•Sperm dynamics in both control and ultrasound-treated specimens were assessed by a CASA system after liquefaction.•Ultrasound exposure sufficiently reduced camel semen viscosity, increased sperm progressive motility, as well as sperm dynamics criteria.•These results point to the beneficial use of this novel approach as a sufficient alternative to enzymatic liquefaction of dromedary camel semen.

The current investigation aimed to evaluate the rheological and physical properties of camel semen subjected to liquefaction by high-power low frequency ultrasound. Ejaculates (n = 18) were collected during the rutting season from 2 adult male camels, Camelus dromedarius, (9 ejaculates each) using a modified artificial vagina. Immediately after collection, each ejaculate was split into 3 aliquots by a split-sample technique. The first aliquot (raw) was evaluated for rheological, physical and morphological properties, whereas the other 2 aliquots were diluted (1:3) with Tris-lactose egg yolk extender. An aliquot of diluted semen served as control and was evaluated for the aforementioned criteria immediately after dilution (T0) and after an equilibration period of 3 h at 5 °C (T180). Meanwhile, the other diluted specimen was exposed to 4 sets of a 40 kHz ultrasound nominal frequency, 2 min each at 2 min interval, at 37 °C for physical viscosity elimination. Throughout the exposure period, semen rheological properties and sperm physical characteristics were evaluated. Thereafter, sperm kinetics of equilibrated control and ultrasonic-treated semen were objectively assessed by a computer-assisted sperm analysis (CASA) unit. Time of exposure to the ultrasonic waves was negatively correlated (P < 0.01) with each of viscosity score (r = −0.95) and thread length (r = −0.93), whereas it was positively correlated (P < 0.01) with the percent of progressive motility (r = 0.87). Furthermore, ultrasonic-treated semen recorded higher (P < 0.05) motility, viability and sperm dynamics criteria than those of control. These results point to the successful application of ultrasound technology to improve handling, evaluation and processing of camel semen.

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