Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5795816 | Small Ruminant Research | 2013 | 5 Pages |
Abstract
A total of 36 four-month-old crossbred lambs (Dorset Ã Thin-tailed Han sheep) with similar BW were randomly allocated to three dietary treatments with different energy (7.21, 10.33 and 13.49 MJ dâ1 ME). Longissimus dorsi, femoral biceps and cardiac muscle tissue were sampled and total RNA was extracted to determine relative transcript levels of the Heart-type fatty acid-binding proteins (H-FABP) gene by real-time PCR. The results showed that the abundance of H-FABP mRNA in different tissues of sheep increased with the elevation of dietary energy. In the longissimus dorsi and femoral biceps muscles, the H-FABP mRNA levels showed significant differences among the three groups (P < 0.01); in the cardiac muscle, the H-FABP mRNA level in the high energy group was significantly higher than that in the moderate and low energy groups (P < 0.01), and the H-FABP mRNA level in the moderate energy group was significantly different from the low energy groups (P < 0.05). The number of H-FABP copies (Qty) in different tissues of sheep was different, it was greater in the cardiac muscle tissue than in longissimus dorsi muscle and femoral biceps muscle.
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Authors
Y.J. Zhang, Y.Q. Liu, J. Song, S.Y. Cheng, L.X. Dong,