Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5796190 | Small Ruminant Research | 2012 | 8 Pages |
Enzyme activity of protein and carbohydrate degradation in small intestinal mucosa was investigated in goat kids fed with lyophilized bovine and goat colostrum. At 0, 7 and 14Â h of life 15 male newborns received 5% of body weight of lyophilized bovine colostrum and 14 goat colostrum, both with 55Â mg/mL of IgG. Duodenum, jejunum and ileum samples were collected at 18, 36 and 96Â h of life. Three animals were sampled at birth, without colostrum intake. Activity of aminopeptidase N and A, dipeptidil peptidase IV, lactase, maltase and sucrase was determined as one international unit per gram of tissue. Intracellular enzymatic activity of acid phosphatase was observed by histochemistry in tissue section. Only the activity of aminopeptidase A in the ileum was affected by treatment, with a greater value for LBC than for GC (PÂ <Â 0.05). The aminopeptidase N activity was the highest at 36Â h in the duodenum (PÂ <Â 0.05) and lowest at 96Â h in the jejunum (PÂ <Â 0.05). Dipeptidil peptidase IV activity was highest at 36Â h in the duodenum (PÂ <Â 0.05), lowest at 96Â h in the jejunum (PÂ <Â 0.05) and higher at 36Â h than at 96Â h in the ileum (PÂ <Â 0.05). Aminopeptidase A activity in the ileum was highest at 36Â h (PÂ <Â 0.05), followed by 18 and 96Â h of life (PÂ <Â 0.05). Lactase activity in the duodenum increased from 18 to 36Â h and from 36 to 96Â h in the jejunum (PÂ <Â 0.05). Maltase activity increased only in the duodenum from 18 to 96Â h (PÂ <Â 0.05). Sucrase activity in the jejunum decreased from 18 to 36Â h and from 36 to 96Â h in the ileum (PÂ <Â 0.05). At birth, activity of most enzymes was similar to that at later times (PÂ <Â 0.05). Histochemistry analyses showed a higher frequency of lysosomes with acid phosphatase activity in the duodenum, especially at 36Â h of life. In the jejunum, the presence of lysosomes with acid phosphatase activity was the highest at 96Â h, followed by 36 and 18Â h of life. In the ileum, all samples showed low presence of lysosomes with acid phosphatase activity. These results indicate that lyophilized bovine colostrum, as a heterologous source of antibodies or nutrients, is a possible alternative management tool for goats. The present work also suggests that in the first 4Â days of life, enzyme activity in the intestinal epithelium of goats is still not fully stimulated, which is an important characteristic for these animals that depend on macromolecule absorption to acquire passive protection after birth.