Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5796612 | Veterinary Immunology and Immunopathology | 2016 | 12 Pages |
Abstract
Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmm strains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; PÂ <Â 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine.
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Authors
Isabel Nkando, Jose Perez-Casal, Martin Mwirigi, Tracy Prysliak, Hugh Townsend, Emil Berberov, Joseph Kuria, John Mugambi, Reuben Soi, Anne Liljander, Joerg Jores, Volker Gerdts, Andrew Potter, Jan Naessens, Hezron Wesonga,