| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5797203 | Veterinary Immunology and Immunopathology | 2007 | 11 Pages |
The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10 exons and 9 introns, with an open reading frame (ORF) of 903Â nt encoding 301Â aa. The IRF-7 gene has 6057Â nt and also contains 10 exons and 9 introns, with an ORF of 1308Â nt encoding 436Â aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-1 and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48Â h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12Â h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48Â h post-stimulation.
