Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5797666 | The Veterinary Journal | 2015 | 6 Pages |
â¢The aim of the present study was to investigate the role of exogenous α-L-fucosidase during fertilisation in pigs.â¢Î±-L-fucosidase increased sperm ZP binding and penetration, suggesting a role of the enzyme in fertilisation ability of sperm.â¢The enzyme increased sperm intracellular calcium concentration and tyrosine phosphorylation, suggesting a role in capacitation.â¢These data indicate that α-L-fucosidase enhances capacitation-associated events in porcine spermatozoa.
The activity of α-L-fucosidase in oviductal fluid increases around the time of ovulation. α-L-fucosidase is also associated with the spermatozoal plasma membrane and its substrate, fucose, has been identified in the zona pellucida (ZP) and on the spermatozoal surface, suggesting a role in fertilisation. The aim of the present study was to investigate the role of exogenous α-L-fucosidase during fertilisation. Porcine oocytes were incubated with fucosidase and later subjected to in vitro fertilisation (IVF). No effect on the percentage of oocytes fertilised was observed, although there was a slight decrease in spermatozoa-ZP binding. Fucosidase was then added to IVF medium, and spermatozoa and oocytes were co-incubated for 15âmin. A significant increase in spermatozoa-ZP binding and penetration was observed, suggesting a role of the enzyme in the fertilisation ability of spermatozoa. In addition, fluorescence intensity and the patterns of spermatozoa membrane-associated α-L-fucosidase distribution, as assessed by indirect immunofluorescence, were not affected by the presence or absence of exogenous enzyme, suggesting an independent role for the exogenous and spermatozoa-associated enzymes. Addition of exogenous α-L-fucosidase increased the spermatozoal intracellular ionised calcium concentration and tyrosine phosphorylation, suggesting a role in promoting capacitation and, at the same time, protecting spermatozoa from a premature acrosome reaction. Thus, α-L-fucosidase enhances capacitation-associated events in porcine spermatozoa.