| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5797891 | The Veterinary Journal | 2014 | 4 Pages |
â¢An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay.â¢On field serum samples the diagnostic sensitivity of the S1 ELISA was 100%, the diagnostic specificity was 94% and the S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses.â¢On colostrum samples, the diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA.â¢The results suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.
An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. In serum samples from experimentally infected pigs (nâ=â35), anti-IgG PEDV antibodies were detected as early as 7 days post-infection. In field serum samples (nâ=â239), the diagnostic sensitivity of the S1 ELISA was 100% and the diagnostic specificity was 94%. The S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples (nâ=â133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.
