| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5798796 | The Veterinary Journal | 2013 | 5 Pages |
Abstract
Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2Â h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
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Authors
Katy Webb, Colin Barker, Tihana Harrison, Zoe Heather, Karen F. Steward, Carl Robinson, J. Richard Newton, Andrew S. Waller,