Whole genomic analysis of porcine G10P[5] rotavirus strain P343 provides evidence for bovine-to-porcine interspecies transmission

•Whole genomic analysis of a porcine G10P[5] rotavirus detected in Thailand.•Strain P343 was found to have a bovine rotavirus-like genotype constellation.•Each of the 11 genes of strain P343 appeared to be of bovine origin.•Strain P343 is likely a bovine rotavirus strain that was transmitted to pigs.

Porcine group A rotavirus (RVA) strain P343 (RVA/Pig-tc/THA/P343/1991/G10P[5]) was suggested to have VP7 and VP4 genes of bovine origin. In order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (VP6, VP1-3, and NSP1-5) of strain P343 were sequenced and analyzed in the present study. On whole genomic analysis, strain P343 was found to have a bovine RVA-like genotype constellation (G10-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3) different from those of typical porcine RVA strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strain P343 appeared to be of bovine origin. Therefore, strain P343 was suggested to be a bovine RVA strain that was transmitted to pigs.