The use of epifluorescence microscopy and fluorescent dyes for visualization of Oxyuris equi eggs

•The results indicate that this new method permits a quick, easy, clear and selective visualization of the Oxyuris equi eggs.•The new fluorescent dyes developed are cost effective and environmentally safe.•The fluorescent dyes used in this study were soluble in water, and had excellent thermal and photochemical stability.•The samples may be stored for future studies without degradation of the dye.

This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe. Other dyes currently used for staining biologic materials use organic solvents, which may be combined with acids or bases in their formulation, preventing their use with the tape method.

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