| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5807096 | Ticks and Tick-borne Diseases | 2015 | 4 Pages |
Abstract
Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2.
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Authors
Gabriele Margos, Sylvia Stockmeier, Cecilia Hizo-Teufel, Sabrina Hepner, Durland Fish, Hans Dautel, Andreas Sing, Eldina Dzaferovic, Melissa Rieger, Sabrina Jungnick, Katrin Binder, Reinhard K. Straubinger, Volker Fingerle,