Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5908361 | Infection, Genetics and Evolution | 2016 | 6 Pages |
Abstract
Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by ε-toxin. A mutated ε-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-ε recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated ε-toxin. This study showed that the LC-pT1NX-ε could be a promising vaccine candidate against the enterotoxemia disease.
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Authors
Mojtaba Alimolaei, Mehdi Golchin, Hamid Daneshvar,