| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5909213 | Infection, Genetics and Evolution | 2015 | 6 Pages |
â¢miRNA and mRNA expression profiles of JEV infected swine testis cells were analyzed.â¢104 known miRNAs and 9 new miRNA candidates were identified.â¢435 known and 94 novel miRNA-mRNA interaction pairs were predicted.â¢miRNAs may play critical roles in the pathogenesis of JEV infection.
To elucidate the role of microRNAs (miRNA) in the regulation of gene expression in Japanese encephalitis virus (JEV) infected swine testis (ST) cells, we analyzed miRNA and mRNA expression profiles of JEV infected ST cells by high-throughput sequencing technology as compared to uninfected controls. The results showed that 104 known miRNAs and 9 new miRNA candidates were differentially expressed in ST cells after JEV infection. We identified 396 differentially expressed mRNAs. Bioinformatics analysis identified 435 known miRNA-mRNA interaction pairs and 94 novel miRNA-mRNA interaction pairs involving miRNAs inversely correlated with the expression of their predicted target mRNAs. The known miRNAs inversely correlated with their target genes were involved in the biological processes of immunity, cytokine production, inflammation, and apoptosis. Selected miRNA-mRNA interactions were validated by luciferase reporter assay. Overall, our findings indicate that miRNAs may play critical roles in the pathogenesis of JEV infection.
