Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5910119 | Infection, Genetics and Evolution | 2014 | 8 Pages |
Abstract
Sahariya, a primitive tribe, native of North Central India, is characterized by a significantly increased incidence of pulmonary tuberculosis (PTB) as compared to other tribes from the same region. Host genetic factors are known to influence susceptibility to PTB at the population level. Since an association of immune regulatory genes, particularly HLA, with PTB susceptibility has already been reported in several studies, we investigated a similar association of HLA alleles with PTB pathogenesis in the Sahariya tribe. A total of 210 cases and 178 healthy individuals from Sahariya tribe were genotyped for HLA class I and II alleles using the PCR based SSP and Reverse-SSO methods. The study showed a significantly increased allelic frequency of HLA-DRB1â15 (p = 0.02) in the patients as compared to healthy controls. However, the allelic frequency of HLA-DRB1â16 was significantly reduced in patients than in controls (p = 0.01). Three locus haplotype analysis of HLA-A, B and DR revealed a significantly increased frequency of Aâ24-Bâ40-DRB1â15 haplotype among patients than controls (p = 0.005), while Aâ02-Bâ40-DRB1â16 and Aâ02-B40-DRB1â03 (p = 0.001 and 0.02, respectively) were significantly reduced in the patients. Our findings confirm a positive association of HLA-DRB1â15 in Sahariya tribe similar to the one already shown in other Indian ethnic groups. On the other hand, HLA-Aâ24-Bâ40-DRB1â15 haplotype was found to be specific to Sahariya tribe with a strong predisposition to PTB. Further, the protection offered by DRB1â16 allele and associated haplotypes towards PTB in this tribe appears to be a novel finding that warrants further investigation with regard to resistance to PTB.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Ecology, Evolution, Behavior and Systematics
Authors
Gunja Mishra, Neeraj Kumar, Gurvinder Kaur, Sanjay Jain, Pramod Kumar Tiwari, Narinder K. Mehra,