| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5910629 | Infection, Genetics and Evolution | 2013 | 7 Pages |
In tropical countries of Asia, like India, approximately 0.5 million children of <5Â years of age die annually due to acute respiratory illness (ARI). Of common respiratory pathogens, influenza viruses (A & B) are associated with annual worldwide epidemics; while the information on influenza C virus is inadequate. During January 2011 through December 2012, 2737 nasal and/or throat swabs were collected from patients reporting at outpatient department of hospitals in eastern India with ARI. Nucleotide sequencing was carried out using gene specific primers followed by pair-wise sequence alignments, multiple alignments, construction of phylogenetic tree and analysis of deduced amino acid sequences. Study reveals that, out of 2737 samples, 1616 (59.04%) were positive for one or more respiratory viruses; of which 23.72% were positive for influenza A and B viruses. From influenza A & B negative samples, influenza C virus was screened and detected with a frequency of 0.18%. Phylogenetic analysis showed that the HE, matrix, NS, PB1 and PB2 gene of the studied strain (C/Eastern-India/1202/2011) possessed a close relatedness to C/Yamagata/26/81 like strains. The P3 gene shows proximity with C/Mississipi/80 like strains whereas NP gene revealed similarity with C/Miyagi/1/93 like strains. The outcome of the whole genome analysis of the strain C/Eastern-India/1202/2011 provided useful information regarding genetic diversity of influenza C strains in India.
⺠Information of endemic strains from Indian subcontinent was largely unknown due to lack of any systemic study. ⺠This is the first report regarding whole genome analysis of influenza C virus from eastern India. ⺠Influenza C virus causes URTI, hence regular surveillance is required. ⺠Genetic lineage identification would offer additional data for epidemiological purpose.
