| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5920045 | Molecular Phylogenetics and Evolution | 2013 | 6 Pages |
Abstract
⺠We develop a Target Restricted Assembly Method for obtaining phylogenomic data from next-generation sequencing reads. ⺠This method uses BLAST searches of reads from a single Illumina lane to identify matching reads for target genes. ⺠Matching reads are assembled locally to produce complete single copy nuclear protein coding sequences. ⺠In an example with 20 species of lice (Psocodea) we recover sequences for 10 genes and perform a phylogenetic analysis.
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Authors
Kevin P. Johnson, Kimberly K.O. Walden, Hugh M. Robertson,