Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5921052 | Physiological and Molecular Plant Pathology | 2015 | 8 Pages |
Abstract
Tomato leaf mold is an important disease caused by Cladosporium fulvum. The Cf-19 gene, which has been shown to confer resistance to this disease in the field, can serve as a new candidate gene in breeding, but the lack of understanding about this gene restricts its application. In this study, we utilized the cDNA-AFLP technique combined with bulk segregant analysis (BSA) to isolate tomato genes related to the Cf-19-mediated resistance response. Twenty-six differentially expressed transcript-derived fragments (TDFs) were successfully isolated based on four expression patterns. Expression patterns of six TDFs were further investigated by qRT-PCR, and the results were consistent with the results of the cDNA-AFLP analysis. Most of the genes detected by transcript profiling of the Cf-19-mediated defense response showed similar expression patterns in the Cf-4 and Cf-9-mediated defense responses. Two genes, SAMDC (TDF35) and Eli3 (TDF49), were first detected in the Cf/Avr interaction. TDF16 showed similarity with a receptor-like protein and the gene cluster Hcr9 where Cf-4 and Cf-9 were located. The genes detected in this study may play important roles in Cf-19-mediated resistance response.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Tingting Zhao, Guan Liu, Shuai Li, Jingfu Li, Jingbin Jiang, He Zhang, Ligong Kang, Xiuling Chen, Xiangyang Xu,