Metabolic network analysis of perfused livers under fed and fasted states: Incorporating thermodynamic and futile-cycle-associated regulatory constraints

► Stoichiometric methodology involving thermodynamic and futile cycle constraints. ► Perfused liver metabolic analysis to compare fed and fasted states. ► Gluconeogenesis, glycogenolysis and fatty acid oxidation are active in both states. ► More pathways with relatively lower weight values are identified in fed state.