Sage oil extraction and optimization by response surface methodology

Sage oil extraction is important as most of the components in sage play an important role in the treatment of various diseases. The aim of the present study was to optimize supercritical extraction parameters for sage (Salvia officinalis L.) oil yield by response surface methodology. A 3-factor Box-Behnken design was used to generate factor combinations. The optimal conditions for the sage oil extraction yield were 280 °C extraction temperature, 110 min extraction time and 11% (wt/v) plant concentration in ethanol. The validation experiment showed that the actual and predicted values were 49.21 and 47.04 wt%, respectively. The composition of sage essential oil consisted mainly of acid esters together with phenols, limonene, indoles, and pyrroles. These compounds can be used in various industrial applications.