Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6378409 | Journal of Stored Products Research | 2014 | 8 Pages |
Abstract
Extraction period and temperature affected the sensitivity of the SPME method. Longer exposure time and higher temperature allowed more analytes to be collected. Consequently, extraction for 4 h at 50 °C appeared to be the optimum condition for extracting pheromones produced by the beetles covered by this study. The method was further adjusted to be more practical and this resulted in changing the sensitivity. Detection of a single R. dominica could still be successfully achieved, even though the time and the temperature were reduced, whereas for the remaining species, the minimum number of insects required had to be increased by about 40-60% in order to detect volatile pheromone in headspace. In conclusion, the SPME technique in detection of infestation is still required further development to be applied to the grain industry. Dynamic headspace may help improve the sensitivity of technique.
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Authors
Wathukan Laopongsit, George Srzednicki, John Craske,