Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6378572 | Postharvest Biology and Technology | 2016 | 7 Pages |
Abstract
Organic acids play a critical role in flavor and overall organoleptic quality of fruit. Malate has been considered to be the major organic acid determining apple fruit acidity. Here, we found that 1-methylcyclopropene (1-MCP) delayed the loss of malate and citrate during storage. 1-MCP-treated fruit showed higher level of phosphoenol pyruvate carboxylase (PEPC) and cytosolic NAD-dependent malate dehydrogenase (cyNAD-MDH) activities, but lower or similar phosphoenolpyruvate carboxylase kinase (PEPCK) and cytosolic NAD-dependent malate dehydrogenase (cyNADP-ME) activities compared to the control. Moreover, the expression of acid transport genes, including MdVHA-A, MdVHP and Ma1, was up-regulated in 1-MCP-treated fruit, resulting in retention of malate in the vacuole, and eventual higher malate content. Hence, 1-MCP maintained fruit acidity by regulating the balance between malate biosynthesis and degradation. These findings provide molecular evidence for understanding the mode of action of 1-MCP regulating organic acid metabolism in apple fruit during storage.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agronomy and Crop Science
Authors
Ruiling Liu, Yuying Wang, Guozheng Qin, Shiping Tian,