Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6378658 | Postharvest Biology and Technology | 2015 | 7 Pages |
Abstract
Ethylene induced significant protein degradation after only 2Â d of exposure, with total protein levels declining by about 60% after 6Â d. The decline in total protein was accompanied by significant increases in protease activity. Increased activity was also observed after 2Â d, reaching levels 9.3-fold higher than initial values after 6Â d. Total protease activity was greatest at pH 5 and declined sharply through pH 8. In-gel assays revealed three ethylene-responsive proteases with apparent molecular masses of 56, 106, and 180Â kDa. Levels of the 106 and 180Â kDa proteases increased after 2Â d of ethylene exposure, reaching maxima at 4-5Â d coincident with symptoms of watersoaking. A 56Â kDa protease was observed only in ethylene-treated fruit and was first detected after 4Â d of ethylene exposure. Together with up-regulated nuclease activities and reactive oxygen species (ROS)-generating capacity, the early appearance of proteases in response to ethylene provides additional evidence that watersoaking in immature cucumber fruit reflects the execution phase of a PCD pathway.
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Authors
Jin Su Lee, Brandon M. Hurr, Donald J. Huber, C.Eduardo Vallejos, Steven A. Sargent,