Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6382083 | Aquatic Toxicology | 2015 | 6 Pages |
â¢European sea bass was exposed to CdCl2 and n-TiO2 alone and in combination.â¢Genotoxicity was evaluated by RAPD-assay, comet assay and cytome assay.â¢CdCl2 induced DNA primary damage but not chromosomal damage.â¢n-TiO2 induced chromosomal damage but not DNA primary damage.â¢Co-exposure effects depend on the biomarker used.
Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2 (1 mg Lâ1) either alone and combined with CdCl2 (0.1 mg Lâ1) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2 and n-TiO2 exposure. Exposure to n-TiO2 alone was responsible for chromosomal alteration but ineffective in terms of DNA damage; while the opposite was observed in CdCl2 exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability.