The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars

In blueberry cultivar Duke, shoot proliferation trials were carried out comparing the effects of 2-isopentenyladenine (2iP) and TDZ; furthermore, experiments of blueberry direct and indirect organogenesis were made, using TDZ as alternatives to zeatin, the most common and expensive hormone used for blueberry in vitro regeneration. Different regeneration responses were observed by using TDZ alone or combined with 2iP. The addition of both 0.2 mg L−1 or 0.5 mg L−1 of TDZ in the medium led to improved callus formation. The addition of 15 mg L−1 of 2iP in the same medium promoted blueberry stem elongation, while inhibiting callus growth. Zeatin was most efficient in direct regeneration of shoots while 0.5 mg L−1 TDZ induced a highest number of shoots by indirect organogenesis.