Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6406657 | Scientia Horticulturae | 2015 | 7 Pages |
Abstract
Identifying the rates of nitrate absorption by almond plants under water stress is important for improving nitrogen use efficiency in almond orchards. This study aimed to determine the rates of nitrate absorption by almond plants subjected to short-term water stress and no water stress, and the kinetic NO3â absorption parameters in well-watered three-year-old almond plants. Two assays were performed. In the first, fourteen plants arranged in completely randomized design received nutrient solutions with water potentials (Ψw) of 0.0, â0.2, â0.4 and â0.7 MPa and containing 1.2 mmol lâ1 KNO3. We accessed the NO3â depletion in these solutions over an 8-h interval and estimated regression curves of the quantity of nitrate in the nutrient solution (Q) as a function of time (t). Subsequently nitrate uptake rates were calculated. Water loss; nitrate reductase activity; nitrate and total N of the sap; nitrate concentration of leaf dry matter; and nitrate and nitrite concentration of root dry matter were, either, evaluated. The second assay was performed the same way of the first one, with four well-watered plants submitted to 0.3 mmol lâ1 KNO3 as initial concentration, and allowed estimating NO3â absorption parameters Vmax, Km and Cmin. We observed a linear decrease in the nitrate content of the containers receiving 1.2 mmol lâ1 of NO3â over a period of 8 h. In this concentration, the rates of NO3â uptake ranged from 1.11 to 3.43 μmol gâ1 hâ1 and decreased in low water potentials at the root medium. Root nitrate reductase activity followed the same trend of nitrate absorption. In the low NO3â concentration range, the absorption showed a Michaelis-Menten pattern, being the kinetic parameters of NO3â absorption 1.15 ± 0.27 μmol gâ1 hâ1, 28.81 ± 4.12 μmol lâ1 and 17.93 ± 1.1 μmol lâ1 for Vmax, Km, and Cmin, respectively. We concluded that NO3â uptake of almonds is affected by short periods of water stress, with harmful effects occurring at Ψw below â0.18 MPa.
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Authors
Herminia E.P. Martinez, Andres Olivos, Patrick H. Brown, Junia M. Clemente, Claudio H. Bruckner, John L. Jifon,