| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6481796 | Stem Cell Research | 2016 | 5 Pages |
Abstract
Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VECÂ + endothelial cells express tdTomato and CD43Â + hematopoietic progenitors express eGFP.
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Authors
Ho Sun Jung, Gene Uenishi, Akhilesh Kumar, Mi Ae Park, Matt Raymond, Dustin Fink, Ethan McLeod, Igor Slukvin,