Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6481803 | Stem Cell Research | 2016 | 4 Pages |
Abstract
We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.
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Authors
Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon D. Hennebold, Kyle E. Orwig, Amander T. Clark,