The effect of disulfide bond introduction and related Cys/Ser mutations on the stability of a cyclohexanone monooxygenase

After verification by ABD-F labeling that this mutant does not form a disulfide bond, single and double Cys/Ser mutants were prepared and investigated. Subsequent analysis revealed that the T415C single point variant is the most stable variant with a 30-fold increased long-term stability (33% residual activity after 24 h incubation at 25 °C) showcasing a great achievement for practical applications.