Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6490832 | Journal of Biotechnology | 2015 | 39 Pages |
Abstract
After verification by ABD-F labeling that this mutant does not form a disulfide bond, single and double Cys/Ser mutants were prepared and investigated. Subsequent analysis revealed that the T415C single point variant is the most stable variant with a 30-fold increased long-term stability (33% residual activity after 24 h incubation at 25 °C) showcasing a great achievement for practical applications.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Sandy Schmidt, Maika Genz, Kathleen Balke, Uwe T. Bornscheuer,