Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6494328 | Metabolic Engineering | 2015 | 12 Pages |
Abstract
Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization, and a synthesis step that fuels additional hypothesis generation. This cycle mirrors the Design-Build-Test-Learn cycle followed throughout various engineering fields that has recently become a defining aspect of synthetic biology. This review will attempt to summarize recent genome-scale design, build, test, and learn technologies and relate their use to a range of metabolic engineering applications.
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Authors
Rongming Liu, Marcelo C. Bassalo, Ramsey I. Zeitoun, Ryan T. Gill,