Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8318025 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2018 | 5 Pages |
Abstract
We examined ionoregulatory characteristics of four Characids from diverse locations in South America, emperor tetras (Nematobrycon palmeri), penguin tetras (Thayeria boehlkei), serpae tetras (Hyphessobrycon eques), and rosy tetras (Hyphessobrycon rosaceous). When held in 100â¯Î¼molâ¯Lâ1 Na+ water, tetras had Jmax values over 1100â¯nmolâ¯gâ1â¯hâ1, and Km values below 60â¯Î¼molâ¯Lâ1. When held in 1â¯mmolâ¯Lâ1 Na+ water kinetic parameters were unchanged. Low pH had no effect on Na+ uptake (JinNa). At pHâ¯3.25, Na+ loss (JoutNa) was stimulated 35-85% in two of the four species. To test the linkage of JinNa to NH3 and H+ extrusion we measured JinNa during exposure to 1â¯mmolâ¯Lâ1 NH4Cl (HEA) and 100â¯Î¼molâ¯Lâ1 Acetazolemide (AZ). HEA stimulated JinNa of emperor tetras by 40%, but inhibited JinNa of penguin tetras by 50%; the two remaining species were unaffected. AZ (an inhibitor of carbonic anhydrase) inhibited JinNa of serpae tetras by 40%, but had no effect on the others. All tetras displayed ionoregulatory characteristics that are very similar to each other, which supports the argument that these physiological traits may be ancestral for this group and pre-date colonization of the Rio Negro. The novel finding that, Jmax and Km did not change after acclimation to 1â¯mM Na+ water indicates that, unlike in other species examined uptake is not plastic. The HEA and AZ results, along with pH insensitivity suggest Na+ uptake is not coupled to H+ extrusion or NH3 excretion and leaves the exact mechanism involved unclear.
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Authors
R.J. Gonzalez, A. Cradeur, M. Guinnip, A. Mitchell, V. Reduta,