Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8318712 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2012 | 7 Pages |
Abstract
The present study aimed to test starvation-induced oxidative stress in the cinnamon clownfish Amphiprion melanopus illuminated by light-emitting diodes (LEDs): red (peak at 630Â nm), green (peak at 530Â nm), and blue (peak at 450Â nm) within a visible light. We investigated the oxidative stress induced by starvation for 12Â days during illumination with 3 LED light spectra through measuring antioxidant enzyme (superoxide dismutase [SOD] and catalase [CAT]) mRNA expression and activity; CAT western blotting; and measuring lipid peroxidation [LPO]), plasma H2O2, lysozyme, glucose, alanine aminotransferase (AlaAT), aspartate aminotransferase (AspAT), and melatonin levels. In green and blue lights, expression and activity of antioxidant enzyme mRNA were significantly lower than those of other light spectra, results that are in agreement with CAT protein expression level by western blot analysis. Also, in green and blue lights, plasma H2O2, lysozyme, glucose, AlaAT, AspAT, and melatonin levels were significantly lower than those in other light spectra. These results indicate that green and blue LEDs inhibit oxidative stress and enhance immune function in starved cinnamon clownfish.
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Authors
Cheol Young Choi, Hyun Suk Shin, Young Jae Choi, Na Na Kim, Jehee Lee, Gyung-Suk Kil,