The antibacterial and anti-inflammatory investigation of Lawsonia Inermis-gelatin-starch nano-fibrous dressing in burn wound

The aim of this study was to develop a new bioactive gelatin-oxidized starch nanofibers containing Lawsonia Inermis (henna) for treating second-degree burn wounds. Continuous, smooth, and bead-free fibers were obtained when the gelatin-starch ratio was 70/30.Additionally, the fiber dimeter was reduced with increasing henna content. The successful loading of henna in the gelatin-oxidized starch nanofibers was approved using Fourier transform infrared spectroscopy and Differential scanning calorimetry. Moreover, the addition of henna to the gelatin-oxidized starch nanomatrix enhanced fibroblasts attachment, proliferation, collagen secretion, and antibacterial activity. In vivo studies showed that the nanofibers loaded with henna accelerated wound closure remarkably with the absence of detrimental suppurative reaction at the site of the burn wound. The CD68 immunohistochemical stained wound images showed that treating the burn wound sites with gelatin-oxidized starch-henna reduced the inflammatory response and macrophage numbers significantly.