Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8329036 | International Journal of Biological Macromolecules | 2018 | 40 Pages |
Abstract
In this study, a sulfated Artemisia sphaerocephala polysaccharide (ASPs) was prepared and its antitumor activity was evaluated in tumor cells and Hepatoma 22 (H22) tumor-bearing mice. In vitro experiments, ASPs significantly inhibited the growth of HepG2 and Hela cells with the IC50 values of 172.03 and 161.42 μg/mL, respectively. Moreover, no direct cytotoxicity against mouse fibroblast L929 normal cells was observed in vitro. After oral administration for 12 days, the tumor growth was significantly suppressed by ASPs at the doses of 200 mg/kg (inhibition rate of 60.85%). Results of tumor histological morphology and cell cycle analysis showed that ASPs could arrest H22 cells at S phase and promote cell apoptosis. Additionally, immunohistochemical analysis demonstrated that ASPs caused the down-regulation of mutant p53 protein expression in a dose-dependent manner. Therefore, these findings proposed new insight into antitumor properties of sulfated polysaccharide as a promising agent in cancer treatment.
Keywords
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Biochemistry
Authors
Junlong Wang, Aijuan Bao, Qi Wang, Hongyun Guo, Yongdong Zhang, Junyu Liang, Weibao Kong, Jian Yao, Ji Zhang,