Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8353167 | Plant Physiology and Biochemistry | 2018 | 43 Pages |
Abstract
Tobacco BY-2â¯cells were used as a model system to address the question of the role of C. herbarum pGM in cell viability and induction of the expression of plant defense-related genes. Native and partially acid hydrolyzed pGMs (lacking galactofuranosyl side-chain residues) were incubated with BY-2â¯cell suspensions at different concentrations. Cell viability drastically decreased after exposure to more than 400â¯Î¼gâ¯mlâ1 pGM; however no cell viability effect was observed after exposure to a partially acid hydrolyzed pGM. BY-2â¯cell contact with pGM strongly induce the expression of plant defense-related genes, such as phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX), as well as the pathogen-related PR-1a, PR-2 and PR-3 genes, suggesting that pGM activates defense responses in tobacco cells. Interestingly, contact with partially hydrolyzed pGM also induced defense-related gene expression at earlier times than native pGM. These results show that the side chains of the (1â¯ââ¯5)-linked β-D-galactofuranosyl units from pGM play an important role in the first line fungus-plant interactions mediating plant responses against C. herbarum. In addition, it was observed that pGM and/or C. herbarum conidia are able to induced HR when in contact with tobacco leaves and in vitro plantlets roots, producing necrotic lesions and peroxidase and NO burst, respectively.
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Plant Science
Authors
Bianca Braz Mattos, Caroline Montebianco, Elisson Romanel, Tatiane da Franca Silva, Renato Barroso Bernabé, Fernanda Simas-Tosin, Lauro M. Souza, Guilherme L. Sassaki, Maite F.S. Vaslin, Eliana Barreto-Bergter,