Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8353439 | Plant Physiology and Biochemistry | 2018 | 10 Pages |
Abstract
Ceratocystis wilt, caused by Ceratocystis fimbriata, is currently one of the most important disease in eucalypt plantations. Plants infected by C. fimbriata have lower volumetric growth, lower pulp yields and reduced timber values. The physiological bases of infection induced by this pathogen in eucalypt plant are not known. Therefore, this study aims to assess the physiological and metabolic changes in eucalypt clones that are resistant and susceptible to C. fimbriata. Once, we evaluated in detail their leaf gas exchange, chlorophyll a fluorescence, water potential, metabolite profiling and growth-related parameters. When inoculated, the susceptible clone displayed reduced water potential, CO2 assimilation rate, stomatal conductance, transpiration rate, photochemical quenching coefficient, electron transport rate, and root biomass. Inoculated resistant and susceptible clones both presented higher respiration rates than healthy plants. Many compounds of primary and secondary metabolism were significantly altered after fungal infection in both clones. These results suggest that, C. fimbriata interferes in the primary and secondary metabolism of plants that may be linked to the induction of defense mechanisms and that, due to water restrictions caused by the fungus in susceptible plants, there is a partial closure of the stomata to prevent water loss and a consequent reduction in photosynthesis and the transpiration rate, which in turn, leads to a decrease in the plant's growth-related. These results combined, allowed for a better understanding of the physiological and metabolic changes following the infectious process of C. fimbriata, which limit eucalypt plant growth.
Keywords
Predawn water potentialmaximum quantum yield of photosystem IINPQRGRETRCi/CaGC–MSGas exchangeanalysis of varianceANOVADark respirationNon-photochemical quenchingPhotochemical quenchingdays after inoculationhours after inoculationgas chromatography–mass spectrometryPrimary metabolismtranspiration rateRelative growth ratesStomata conductanceWater potentialChlorophyll a fluorescence
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
André Costa da Silva, Franklin Magnum de Oliveira Silva, Jocimar Caiafa Milagre, Rebeca Patricia Omena-Garcia, Mário Castro Abreu, Reginaldo Gonçalves Mafia, Adriano Nunes-Nesi, Acelino Couto Alfenas,