Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8354597 | Plant Physiology and Biochemistry | 2015 | 11 Pages |
Abstract
Glutathione reductase (GR; EC 1.8.1.7) is an important oxidoreductase that can protect organisms against various oxidative stresses. In this study, a new GR gene, named as HbGR2, was isolated from Hevea brasiliensis. The HbGR2 cDNA contained a 1674-bp open reading frame encoding 557 amino acids and the deduced HbGR2 protein showed high identities to the chloroplastic GRs from other plant species. HbGR2 was localized in the chloroplasts of tobacco mesophyll protoplasts. The cis-acting regulatory elements related to stress or hormone responses were predicted in the promoter region of HbGR2. The results from real-time RT-PCR analyses showed that HbGR2 was expressed throughout different tissues and developmental stages of leaves. Besides being related to tapping panel dryness (TPD), HbGR2 was regulated by several treatments including ethephon (ET), methyl jasmonate (MeJA), drought, low temperature, high salt, wounding and hydrogen peroxide (H2O2). The Escherichia coli (E. coli) cells overexpressing HbGR2 markedly increased their tolerance and survival at high concentrations of H2O2, suggesting that HbGR2 might play an important role in oxidative stress response in Hevea brasiliensis.
Keywords
isopropyl-ß-d-thiogalactopyranosideABACTABRT-PCRHevea brasiliensisflavin adenine dinucleotideIPTGORFcDNAComplementary DNANADP(H)reverse-transcription PCRESTabscisic acidsodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGEFADexpression sequence tagrapid amplification of cDNA endsOxidative stressbase pairsopen reading frameSubcellular localizationRaceUTR یا untranslated regions untranslated regionNADHHexadecyltrimethylammonium bromidepolymerase chain reactionPCRExpression profilesoptical densityreduced nicotinamide adenine dinucleotidereduced nicotinamide adenine dinucleotide phosphateglutathione reductaseGibberellin
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Authors
Zhi Deng, Manman Zhao, Hui Liu, Yuekun Wang, Dejun Li,