Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8357707 | Plant Science | 2015 | 12 Pages |
Abstract
Faster or stronger response to pathogen occurs if plants undergo prior priming. Cytokinins seem to be also involved in plant priming and in response to pathogens. Susceptibility to Potato virus YNTN (PVYNTN) was studied in transgenic cytokinin overproducing (Pssu-ipt) tobacco and compared with nontransgenic plants. Since cytokinin overproduction inhibits development of plant roots and grafting overcomes this limitation, both types were grown as rooted and/or grafted plants to check also the effect of grafting. Control rooted tobacco (C), the most susceptible to PVYNTN, showed always symptoms during the infection together with the rising virus content and a systemic response, such as accumulation of H2O2, salicylic acid (SA) and other phenolic acids, and stress-induced enzyme activities. In transgenic and grafted plants, the response to PVYNTN was dependent on protective mechanisms activated prior to the inoculation. In Pssu-ipt tobacco, cytokinin active forms and SA contents exceeded manifold their content in C. Grafting promoted the accumulation of phenolics, but SA, and stimulated peroxidase activities. Thus, the pre-infection barrier established in both transgenic and grafted plants helped to suppress partly the virus multiplication and resulted in milder symptom development. However, only the synergic effect of both grafting and the high cytokinins led to PVYNTN tolerance in transgenic grafts. Possible mechanisms were discussed.
Keywords
DABPssuSPODGPODPPDKT/CNADP-MECKXAOEPEPCTMVIPTIBRDAINBTCAT3,3-diaminobenzidineC/CROSnitroblue tetrazoliumNADP-malic enzymemalic enzymeAntioxidant enzymesApoDSalicylic aciddays after inoculationSODsyringaldazine peroxidaseSuperoxide dismutaseCMVcytokinincytokinin oxidase/dehydrogenaseCytokininsAntioxidant systemPhosphoenolpyruvate carboxylaseTobacco mosaic virusCucumber mosaic virusPeroxidaseascorbate peroxidasePyruvate orthophosphate dikinaseCatalaseglutathione reductaseguaiacol peroxidaseReactive oxygen species
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Plant Science
Authors
Petra Spoustová, Veronika Hýsková, Karel Müller, Renata Schnablová, Helena RyÅ¡lavá, Noemi ÄeÅovská, JiÅà Malbeck, Milena Cvikrová, Helena Synková,