Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8358250 | Plant Science | 2014 | 5 Pages |
Abstract
The molecular function of an isoform of sucrose phosphate synthase (SPS) in rice, OsSPS1, was investigated using gene-disruption mutant lines generated by retrotransposon insertion. The progeny of the heterozygote of disrupted OsSPS1 (SPS1+/â) segregated into SPS1+/+, SPS1+/â, and SPS1â/â at a ratio of 1:1:0. This distorted segregation ratio, together with the expression of OsSPS1 in the developing pollen revealed by quantitative RT-PCR analysis and promoter-beta-glucuronidase (GUS) fusion assay, suggested that the disruption of OsSPS1 results in sterile pollen. This hypothesis was reinforced by reciprocal crosses of SPS1+/â plants with wild-type plants in which the disrupted OsSPS1 was not paternally transmitted to the progeny. While the pollen grains of SPS+/â plants normally accumulated starch during their development, pollen germination on the artificial media was reduced to half of that observed in the wild-type control. Overall, our data suggests that sucrose synthesis via OsSPS1 is essential in pollen germination in rice.
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Authors
Tatsuro Hirose, Yoichi Hashida, Naohiro Aoki, Masaki Okamura, Madoka Yonekura, Chikara Ohto, Tomio Terao, Ryu Ohsugi,