Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8358251 | Plant Science | 2014 | 13 Pages |
Abstract
Despite the external application of hydrogen sulfide (H2S) conferring plant tolerance against various environmental cues, the physiological significance of l-cysteine desulfhydrase (L-DES)-associated endogenous H2S production involved in salt-stress signaling was poorly understood. To address this gap, the participation of in planta changes of H2S homeostasis involved in alfalfa salt tolerance was investigated. The increasing concentration of NaCl (from 50 to 300 mM) progressively caused the induction of total l-DES activity and the increase of endogenous H2S production. NaCl-triggered toxicity symptoms (175 mM), including seedling growth inhibition and lipid peroxidation, were alleviated by sodium hydrosulfide (NaHS; 100 μM), a H2S donor, whereas aggravated by an inhibitor of l-DES or a H2S scavenger. A weaker or negative response was observed in lower or higher dose of NaHS. Further results showed that endogenous l-DES-related H2S modulated several genes/activities of antioxidant defence enzymes, and also regulated the contents of antioxidant compounds, thus counterbalancing the NaCl-induced lipid peroxidation. Moreover, H2S maintained K+/Na+ homeostasis by preventing the NaCl-triggered K+ efflux, which might be result form the impairment of SKOR expression. Together, our findings indicated that endogenous H2S homeostasis enhance salt tolerance by coupling the reestablishment of redox balance and restraining K+ efflux in alfalfa seedlings.
Keywords
H2DCF-DAMBBRUPLCPAGdehydroascorbateNaHSTBARSMDHARdehydroascorbate reductaseDHARdl-propargylglycineGSHN-ethylmaleimideTCANMTDTTIRKmonodehydroascorbate reductaseCHESCATMedicago sativaHomoglutathioneASAl-Cysteine desulfhydraseEDXICP-OESH2S2′,7′-dichlorofluorescein diacetateROSAscorbic acidOxidative damagetrichloroacetic acidSalt tolerancePotassium effluxDHAdithiothreitolSODHydrogen sulfideSuperoxide dismutaseInductively Coupled Plasma-Optical Emission Spectrometerthiobarbituric acid reactive substancesMonobromobimaneNEMNitric oxideRedox homeostasisSodium hydrosulfideHypotaurineCatalaseGlutathioneglutathione reductaseGORKReactive oxygen species
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Agricultural and Biological Sciences
Plant Science
Authors
Diwen Lai, Yu Mao, Heng Zhou, Feng Li, Mingzhu Wu, Jing Zhang, Ziyi He, Weiti Cui, Yanjie Xie,