Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8358252 | Plant Science | 2014 | 9 Pages |
Abstract
RNA silencing plays important roles in the regulation of gene expression in eukaryotes. We previously reported that RNA silencing of a linked endogenous gene and a transgene in transgenic rice seeds can be induced by the expression of foreign recombinant mGLP-1, which acts as a silencing-inducible sequence through RNA interference. In this study, we found that the induction of RNA silencing by foreign transgenes is not restricted to mGLP-1 but is observed in many other genes as a relatively general phenomenon, as several foreign genes were involved in inducing RNA silencing in the same manner as mGLP-1 in transgenic rice. We detected 21-24-nt siRNAs using both sense and antisense probes specific to the silenced genes in both the leaves and endosperm of transgenic rice plants. Moreover, read-through transcripts were consistently observed in silenced transgenic rice plants. Taken together, these results suggest that proper transcription termination was prevented in these plants, and the highly divergent 3â²-end transcripts served as templates for double-stranded RNA synthesis, resulting in the degradation of the target genes via siRNA.
Keywords
PTGSOryza sativa L.DCLdsRNARNA interferenceSmall interfering RNAdouble stranded RNARNAisiRNATGsArgonauteSDS-polyacrylamide gel electrophoresisSDS-PAGEtransgeneRNA silencingpost-transcriptional gene silencingtranscriptional gene silencingRISCCo-suppressionendoplasmic reticulumRNA-Induced Silencing ComplexUTR یا untranslated regions untranslated regionAGO
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Lijun Yang, Taiji Kawakatsu, Yuhya Wakasa, Masato Yoine, Fumio Takaiwa,