| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8358750 | Plant Science | 2012 | 11 Pages |
Abstract
⺠Nonplant myrosinases lack known glycosylation and disulfide bridges of plant myrosinases. ⺠T-DNA insertion mutants were used to individually purify nearly identical isoenzymes. ⺠Two native myrosinase isoenzymes with posttranslational modifications were purified. ⺠TGG1 is soluble and TGG2 is insoluble in initial crude extracts prepared with a low salt buffer. ⺠Myrosinase is active in a range of temperature and pH conditions, and after deglycosylation.
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Agricultural and Biological Sciences
Plant Science
Authors
Changhe Zhou, James G. Tokuhisa, David R. Bevan, Asim Esen,