Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8363452 | Soil Biology and Biochemistry | 2016 | 10 Pages |
Abstract
Plant uptake of dissolved organic nitrogen (DON) has been proposed to explain inconsistency in the ecosystem N balance of semi-arid systems. Nevertheless, direct evidence for an ecologically important role of DON in plant nutrition in these systems remains elusive under field conditions. Here, natural abundance 15N signatures of NO3â, NH4+, DON and whole plants from a semi-arid model forest were analyzed to provide robust estimates of plant N source partitioning and relative N cycling rates under in-situ conditions. Concurrently, architectural and symbiotic root traits were determined to assess their relationship to plant N acquisition strategies. Bayesian isotope mixing models indicated an insignificant contribution of DON to ecosystem plant N nutrition. Nitrate was the dominant plant N source in this ecosystem, while the contribution of NH4+ to plant nutrition varied between herbaceous (26%) and woody plants (8%). The low C:N ratio of the dissolved organic matter pool - ranging from 12.7 to 4.9 within the soil profile - indicated microbial C-limitation in this ecosystem. Dissolved organic N was significantly enriched in 15N relative to NH4+ and NO3â, corroborating the importance of dissolved organic matter recycling as a cost-effective pathway that simultaneously supplies C and nutrients for microbial metabolism. Plants exclusively depend on inorganic N forms made available through microbial N mineralization and free-living atmospheric N2 fixation, followed by autotrophic nitrification.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Soil Science
Authors
D. Huygens, S. DÃaz, C. Urcelay, P. Boeckx,