Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8364416 | Soil Biology and Biochemistry | 2015 | 5 Pages |
Abstract
We evaluated the impacts of a macronutrient deficiency on the community structure of root-associated bacteria in potato (Solanum tuberosum) cultivation. Potato plants were cultivated in a long-term experimental field under a nitrogen, phosphorus, or potassium deficiency, and roots were sampled at the early flowering stage. Amplicon libraries of 16S rRNA gene were constructed for root-associated bacteria, and bacterial diversity was analyzed by means of pyrosequencing. Statistical analyses showed significantly lower species evenness in the nutrient-deficient plots compared with the value in a plot with standard fertilization. Phylogenetic analyses revealed that the abundance of Rhizobium spp. increased dramatically in all plots under a macronutrient deficiency.
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Authors
Yusuke Unno, Takuro Shinano, Kiwamu Minamisawa, Seishi Ikeda,